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Diagnosing HIV

Updated on February 02, 2020

Article written by
Kelly Crumrin

The process for diagnosing HIV involves one or more blood or saliva tests. If results are inconclusive, the doctor may order more tests. For tests to be effective and conclusive, it is necessary to wait a certain period of time for HIV to replicate and reach a detectable level. The earliest reliable test for HIV is effective within one to four weeks after exposure. Nearly all tests are reliable about three months after infection.

The time that elapses between exposure and eligibility for testing is called the window period. During the window period, HIV is replicating within the body, creating molecules that can be identified by diagnostic tests. Testing too soon may provide false negative or inconclusive results. During the window period, if you have been infected, you are very contagious.

Anyone who knows or suspects they have been exposed to HIV should be tested as soon as possible and avoid behavior that might risk spreading HIV to others in the meantime. If you are concerned you may have recently been exposed to HIV, talk to your doctor about post-exposure prophylaxis (PEP), a treatment regimen that can help prevent you from becoming infected.

Who Should be Tested for HIV?

Starting HIV treatment early gives people with HIV the best chance for long, healthy lives and also reduces the risk for spreading HIV. For these reasons, the Centers for Disease Control and Prevention (CDC) recommends that everyone between the ages of 13 and 64 be tested for HIV at least once. They also recommend screening all pregnant women for HIV. The CDC recommends annual testing for people who:

  • Are men who have ever had sex with another man
  • Have had anal or vaginal sex with an HIV-positive person
  • Have had more than one partner since their last HIV test
  • Inject drugs and share needles or other equipment with another drug user
  • Have sold sex for money or drugs
  • Have ever had a sexually transmitted infection (STI)
  • Have ever been diagnosed with hepatitis or tuberculosis
  • Have had sex with anyone who has done any of the above, or whose history you do not know

For gay or bisexual men who are sexually active, the CDC recommends testing for HIV every three to six months.

How Is HIV Diagnosed?

HIV is diagnosed by taking samples of blood or saliva and analyzing them for molecules related to HIV.

Tests for HIV

Different types of HIV tests look for different molecules that can provide evidence of infection. Some HIV tests are not conclusive for weeks or months after a person is infected, while others can be used sooner. Note that any HIV test may return a false negative result if a test is taken too early.

Viral Load Tests

The earliest tests that are effective for diagnosing HIV are viral load tests, which can be used between one to four weeks after exposure. One type of viral load test is the RNA or nucleic acid test (NAT). Viral load tests can quantify the amount of HIV genetic material carried in the blood. Viral load tests require a blood sample.

The polymerase chain reaction (PCR) test is another early test that can diagnose HIV and determine viral load. PCR tests can be effective within two to three weeks of exposure.

RNA tests are generally used to confirm an inconclusive HIV diagnosis and monitor the progress of the disease and how effective treatment is at reducing the viral load. PCR tests are used to monitor treatment effectiveness in HIV-positive people and to test babies of HIV-positive mothers shortly after birth. Viral load tests tend to be more expensive than other types of HIV tests.

Rapid Antibody/Antigen Combination Test

Also known as a fourth-generation HIV test, rapid antibody/antigen tests look for both fragments of the virus known as p24 antigen as well as HIV antibodies – a type of protein produced by the body to try to fight HIV. Rapid antibody/antigen tests are considered to become effective between 12 and 45 days after HIV exposure.

Rapid antibody/antigen combination tests are considered to be nearly 100 percent effective at identifying HIV in the chronic/latent stage and about 80 percent effective at diagnosing early infections.

ELISA Test

The enzyme-linked immunosorbent assay, or ELISA test, only checks for HIV antibodies. The ELISA test is nearly 100 percent effective at diagnosing HIV in the chronic/latent stage – a few months after a person is infected – but may miss HIV infections in the acute or early stage within the first two to three months. If your ELISA test result is negative, but you strongly suspect you have been exposed to HIV, it is recommended to get another test after waiting one to three months.

If an ELISA test is positive for HIV, the clinician may perform a HIV-1/HIV-2 differentiation immunoassay or a Western blot test to confirm the infection.

HIV-1/HIV-2 Differentiation Immunoassay

A HIV-1/HIV-2 differentiation immunoassay is most often performed to confirm a positive ELISA test result and identify different HIV strains. The blood test provides very accurate results in less than 20 minutes.

Western Blot Test

Western blot tests are performed less often than they were previously because results took several days to return, and the blood test failed to identify some strains of HIV. Western blots become effective about two months after HIV exposure. Western blots are mainly performed to confirm positive ELISA test results.

Home HIV Testing

There are two home HIV test kits available that are approved by the U.S. Food and Drug Administration (FDA).

OraQuick is performed at home using saliva. Results are available within about 20 minutes. OraQuick is effective about three months after exposure to HIV. About 1 in 12 people who take OraQuick receive a false negative result – the result says they are HIV-negative, when in fact they are HIV-positive.

Home Access HIV-1 allows you to collect a blood sample at home using a finger-prick and mail it to a lab for anonymous testing. Home Access HIV-1 is effective three to six months after exposure to HIV. Results are available by phone within seven business days of mailing in the blood sample. The results of Home Access HIV-1 are considered to be extremely reliable.

How Is AIDS Diagnosed?

Acquired immune deficiency syndrome (AIDS) is the third and final stage of HIV. AIDS is diagnosed under any of the following conditions:

  • CD4 cell count falls below 200 cells per cubic millimeter (cells/mm³)
  • CD4 cells make up less than 14 percent of lymphocytes (a variety of white blood cells)
  • The presence of one or more opportunistic infections such as candidiasis (yeast infection) in the throat or lungs, toxoplasmosis of the brain, Kaposi’s sarcoma, invasive cervical cancer, or many others

Condition Guide

Resources

External resources

myHIVteam resources

Kelly leads the creation of content that educates and empowers people with chronic illnesses. Learn more about her here.

A myHIVteam Member said:

Wow i didn't realize they were that many tests in 2016 i was sick 7 -8 months losing my hair,losing weight , couldn't keep anything down,felt like the… read more

posted 5 days ago

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